Biosynthesis of nanoparticles is under exploration is due to wide biomedical applications and research interest in nanotechnology. Bioreduction of silver nitrate (AgNO3) used for the synthesis of silver nanoparticles respectively with the plant extract; Coleus forskohlii roots extract (Lamiaceae). The plant extract is mixed withAgNO3, incubated and studied synthesis of nanoparticles using UV–Vis spectroscopy. The nanoparticles were characterized by X-ray diffraction (XRD), FTIR, SEM equipped with EDS. The silver nanoparticles synthesized were generally found to be needle in shape with 82.46 nm. The results showed that the roots extract of Coleus forskohlii is very good bioreductant for the synthesis of silver nanoparticles and synthesized nanoparticles active against clinically isolated human pathogens 7 Bacteria and 5 fungus.