Abstract

Development and validation of UV spectrophotometric method for estimation of Deflazacort in bulk drug and Pharmaceutical formulation

An Uv spectrophotometric method for the quantitative determination of Deflazacort, having a highly potent anti-inflammatory and immunosuppressive action, in tablets was developed in present work. The parameters linearity, accuracy, precision, limit of detection limit of quantitation, range were studied according to International Conference on Harmonization guidelines. UV spectroscopic determination was carried out at an absorption maximum of 244 nm nm using methanol as solvent. Results of the analyses were validated statistically and by recovery studies. The proposed method is simple, rapid, precise and accurate and can be used for the reliable quantitation of deflazacort in pharmaceutical formulation. In the UV spectroscopic method linearity over the concentration range of deflazacort was found to be 1-9 ug/ml with a correlation coefficient 0.8979.


Author(s): Anindya Bagchi, P. Mukherjee, Indeerjet kaur, Ramandeep Singh, Alok Semwal

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