The present study was designed to evaluate the antimicrobial efficacy of crude extract of the leaves and stems of Amaranthusspinosus L., Capparisdeciduas (kair), Chenopodium album (bathua) and Salvadorapersica(meswak/jal)against pathogenic microbial strains Bacillus subtilis, E.coli, Bacillus amyloliquefaciens, Staphylococcus aureus, Staphylococcus epidermidisand Streptococcus mutansassayed by using agar well diffusion assay. Four different extracts (acetone, benzene,methanol and cow urine) of each plant were used during the study. The significant results were obtained by all solvent extracts except cow urine extracts on tested pathogens using Agar well diffusion method. Acetone extract revealed strongest antibacterial activity on E.coliand methanol leaf extract showed strongest antibacterial activity on Staphylococcus epidermidis.Preliminarily phytochemical investigation of the crude extract of the leaves and stems of plantsshowed the presence of tannin, alkaloids, glycoside, terpenoids, flavonoid, steriods and saponin. The presence of these secondary metabolites indicates the pharmacological property of the plant leaves and stem. On the basis of this finding, the extracts demonstrating antimicrobial efficacy could result in the discovery of novel antimicrobial agents.
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