Dryopteris filix-mas (L.) Schott traditionally used for the treatment of various ailments by the indigenous people of the hills areas in Bangladesh. So, our present study was designed to evaluate the antioxidant and cytotoxic activities of methanolic extract of leaves. A dose dependent scavenging activity of DPPH radical was observed with good reducing power of the extract. In DPPH radical scavenging assay, the IC50 value of the extract was 275.50μg/ml while the IC50 value for the reference ascorbic acid was 110.13μg/ml and good reducing power activity showed by the extract with increasing the concentration and a total phenol content in the was 238.10 mg/gm of gallic acid and was found to contain 126.80mg/gm of quercetin in flavonoid assay. The extract also possesses potent cytotoxic activity with LC50 25.94μg/ml where the LC50 value of standard vincristine sulphate was 10.00μg/ml.