The petroleum ether, ethyl acetate and aqueous extracts of oldenlandia biflora and pergularia daemia were examined for possible sources of antibacterial activities and phytochemical constituents. Antibacterial activity of the extracts was determined by cup plate method on nutrient agar medium. Two gram- positive bacteria (Bacillus subtilis, Staphylococcus aureus) and four gram – negative bacteria (Pseudomonas epidermis, Escherichia coli, Shigella dysenteriae and Salmonella typhi) are used as test organisms. Oldenlandia biflora extracts showed better activity than the Pergularia daemia extracts. Both the plant extracts exhibited more activity against Bacillus subtilis and Staphylococcus aureus. The ethyl acetate extracts of both the plants exhibited more antibacterial activity compared to petroleum ether and aqueous extracts. Pergularia daemia dose not exhibited antibacterial activity against the Shigella dysenteriae. Both the plants were found to contain alkaloids, tannins and flavonoids.
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