Boerhaavia diffusa is used in Ayurvedic medicine system to treat various health problems. Present work deals with analysis of the antiangiogenic properties of B. diffusa extracts by chicken chorioallantoic membrane (CAM) assay in vivo. The extracts of B. diffusa prepared in acetone, alcohol and benzene were tested administering at 48, 72 and 96 hrs of incubation to observe angiogenesis of the CAM at 144 hrs of development. These extracts of B. diffusa reduced neovascularization. Acetone- extract showed highest inhibitory activity in angiogenic response; followed by benzene and alcohol extracts. The quantitative and microscopic analysis indicate that these results indicated inhibition elongation and proliferation of both secondary and tertiary vessels. It seem to be consequences of interference of extracts in a) signaling of angiogenic agents from epithelial cells or b) cellular apoptosis, which in its absence results in normal CAM angiogenesis. This observations support strong claims of antiangiogenic ethnomedical properties of this plant.
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